Targeting low-voltage-activated calcium channels for neurological and cardiovascular disorders
T-type calcium channels (TTCCs, also referred to as "low-voltage-activated" calcium channels), which include the subtypes Cav3.1 (CACNA1G), Cav3.2 (CACNA1H), and Cav3.3 (CACNA1I), open at negative membrane potentials (−70 to −50 mV). Because of this distinguishing property, they are. These channels play key roles in regulating neuronal excitability, hormone secretion, smooth muscle contraction, and potentially in cardiac pacemaking. They are increasingly recognized as important drug targets due to their broad involvement in various physiological and pathological processes. Inhibitors of Cav3.x are potential drug candidates to treat epilepsy (particularly absence seizures) and neuropathic pain, cardiovascular disorders and may have applications in oncology. This, in turn, carries a risk of inhibitors causing adverse effects on a wide variety of organ systems.
ChanPharm offers comprehensive screening studies on Cav3.1 (CACNA1G), Cav3.2 (CACNA1H), and Cav3.3 (CACNA1I). We utilize both automated high-throughput systems and traditional manual patch clamp setups to deliver highly reliable data for selectivity profiling and hit validation.
A range of inhibitors have been developed to target TTCCs for clinical and experimental use:
SyncroPatch 384
Ion channel screening at scale.
Our high-throughput automated patch clamp platform is fully optimized for large-scale screening of TTCC inhibitors across large compound libraries.
Best suited for: Primary screening, hit confirmation, and robust concentration-response profiling.
NANION TECHNOLOGIES HIGH THROUGHPUT
Patchliner
High-quality functional profiling with automation.
Provides medium-throughput automated patch clamp recordings with excellent gigaseal quality, essential for detailed secondary screening and selectivity profiling against off-target Cav channels.
Best suited for: Secondary screening, lead optimization, and selectivity studies.
NANION TECHNOLOGIES MEDIUM THROUGHPUT
Manual Patch Clamp
Gold-standard ion channel electrophysiology.
Manual patch clamp provides unmatched experimental control and resolution for studying voltage-dependent channel kinetics. This approach is used for deep mechanistic studies and validation of screening results.
Best suited for: Detailed kinetics, complex protocols, and mechanism-of-action studies.
CONVENTIONAL SYSTEMS GOLD STANDARD